A novel experimental porcine model to assess the impact of differential pulmonary blood flow on ischemia–reperfusion injury after unilateral lung transplantation

Abstract Background Primary graft dysfunction (PGD) remains a major obstacle after lung transplantation. Ischemia–reperfusion injury is known contributor to the development of PGD following We developed novel approach assess impact increased pulmonary blood flow in large porcine single-left transplantation model. Materials Twelve left transplants were divided two groups ( n = 6, low- (LF) and high-flow (HF) group). Donor lungs stored for 24 h on ice, followed by In HF group, recipient animals observed 6 reperfusion with partially clamping right artery achieve higher (target 40–60% total cardiac output) transplanted compared LF where was not clamped. Results Survival at 100% both groups. Histological, functional biological assessment did significantly differ between during first reperfusion. also present native showed signs compatible pathophysiological hallmarks ischemia–reperfusion injury. Conclusions Partial animal setting study low versus high ischemia feasible. our study, differential had no effect IRI. However, findings might future studies extracorporeal devices represent specific intra-operative problem bilateral sequential single-lung